However, some droplet digital PCR (ddPCR) and chip-based digital PCR (cdPCR) instruments employ lasers as the fluorescence excitation light source.Īnother variable is the mode of detection. Most instruments use LEDs as light sources to “excite” the fluorophores, generating the fluorescence signal. Coupled with the high out-of-band blocking (≥ OD6) and extremely steep transitions from high transmission to deep blocking, these filters deliver the highest available signal-to-noise ratios.ĭifferent approaches have been used by manufacturers of PCR instruments to detect the fluorescence signals associated with the amplified genetic sequences. The high degree of spectral precision ensures reproducible, reliable results, allowing for six or more distinct fluorescence channels. This allows for minimizing spectral overlap - which is always present between fluorescent probes - and optimizing the filter pass bands. Our technical staff not only have a deep understanding of fluorescence, managing light, and biophotonics but also have extensive experience working with PCR investigators and engineers.Ĭhroma’s ET filters for fluorescence applications such as PCR provide unmatched levels of spectral precision. This has provided Chroma with valuable experience and application expertise. The value of this approach is that only the specific target sequences must be amplified, without needlessly amplifying all the DNA in an entire gene or chromosome.Ĭhroma Technology has been supplying high-quality PCR filters to major test equipment manufacturers for many years. The goal of PCR is to make enough of the target DNA region so that its presence can be measured or confirmed for diagnostic, clinical, or experimental purposes. If the target is an RNA sequence, then DNA that is complementary to the RNA is amplified, as is the case with RNA viruses such as the coronavirus responsible for COVID-19. If the target DNA sequence from a pathogen of interest is detected in a sample from a patient and subsequently amplified, its presence is confirmed by a fluorescence signal. PCR is used in a multitude of applications, including infectious disease diagnosis. DNA must be amplified so that there is enough material to reliably detect and identify it with a high degree of confidence. PCR is a technique to make multiple copies of a short sequence of target DNA, exponentially amplifying it. Filters for Polymerase Chain Reaction (PCR) With our line of PCR Sputtered optical filters, Chroma Technology supplies key components of polymerase chain reaction (PCR) laboratory and clinical test solutions, including digital PCR (ddPCR) and quantitative PCR (qPCR) platforms.

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